Grizzly bear 399, a famous and beloved resident of Grand Teton National Park, was tragically killed in a vehicle collision on Highway 26/89 in Snake River Canyon, Wyoming. Known as the oldest reproducing female grizzly in the Greater Yellowstone Ecosystem, she inspired countless visitors and conservationists with her frequent appearances near park roads, often with her cubs. The 28-year-old bear's identity was confirmed through microchips and ear tags, and she had a yearling cub with her at the time of the accident, whose whereabouts remain unknown. Her death has sparked mourning and calls for enhanced wildlife protection measures to prevent such incidents, as vehicle collisions with wildlife remain a significant issue in the region. Grizzly 399 leaves behind a legacy of over 20 cubs and grand cubs and has been celebrated in documentaries and photography books. The U.S. Fish and Wildlife Service, along with state and local agencies, are investigating the incident.