A recent study has revealed that healthcare workers (HCWs) in India are at a significantly higher risk of contracting tuberculosis (TB) compared to the general population, with an average prevalence of 2,391.6 cases per 100,000 HCWs versus 300 cases per 100,000 in the general populace. This systematic review and meta-analysis, conducted by a team of medical experts from various institutions, highlights the urgent need for improved protective measures and infection control in healthcare settings, especially given the presence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of TB. Laboratory technicians, nurses, and doctors are identified as particularly high-risk roles, with laboratory technicians exhibiting the highest prevalence at 6,468.31 cases per 100,000. The study also emphasizes that inadequate ventilation and inconsistent use of personal protective equipment (PPE) contribute to the elevated TB risk among HCWs. India accounts for approximately one-fourth of the global TB burden, underscoring the critical nature of this public health issue. Overall, the findings call for urgent action to address systemic vulnerabilities within the healthcare infrastructure to protect those on the front lines.